L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒

L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒

1) 英文:L-Glutamic Acid (L-Glutamate/MSG) Assay Kit;

2) 中文:L-谷氨酸[谷氨酸盐/谷氨酸酯/味精/谷氨酸单钠]检测试剂盒;

3) 规格: 60;

4) 货号:K-GLUT

商品说明:
The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Suitable for manual, auto-analyser and microplate formats.
Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

Principle:
(beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD+ + H2O ↔ 2-oxoglutarate + NADH + NH4+
(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 60 assays (manual) / 600 (microplate)
/ 700 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 9 min
Detection limit: 0.21 mg/L
Application examples:
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO, GOST
and NMKL
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Glutamate dehydrogenase solution stable at -20°C
  • No wasted diaphorase solution (stable suspension supplied)
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

爱尔兰Megazyme酶 Enzymes

爱尔兰Megazyme酶 Enzymes

       爱尔兰Megazyme是一家全球领先的制造和供应高品质和创新的检测谷物,食品,饲料,发酵,乳制品和葡萄酒行业的技术。Megazyme成立于1988年,该公司目前提供了超过70种诊断试剂盒和超过300种其他试剂和底物。

该公司产品分以下系列:

1. 生物及食品酶法分析试剂盒 (Diagnostic Kits)
2. 试剂混合物(Reagent Mixtures)
3. 协同因子(Cofactors)
4. 糖酶片剂试验(Carbohydrase Tablet Tests)
5. 蛋白酶片剂试验(Protease Test Tablets)
6. 可溶性呈色底物(Soluble Chromogenic Substrates)
7. 不可溶性(交联的)呈色底物(Insoluble (Crosslinked) Chromogenic Substrates)
8. 酶(Enzymes)
9. 植物凝血素(Lectins)
10. 多聚糖(Polysaccharides)
11. 低聚糖(Oligosaccharides)
12. 相关产品 (General)

 英文名称  酶中文名 规格(酶活性Units) 货号
Acetate Kinase(E.coli) 醋酸激酶[大肠杆菌] 100000 E-ACKEC
RecAcetyl-CoA ynthetase (B.subtilis) Acetyl-CoA合成酶[枯草杆菌] 250 E-ACSBS
Adenylate Kinase (Myokinase)(prokaryote) 转化腺苷酸激酶[肌激酶][原核生物] 10000 E-AMPK
Alcohol dehydrogenase(E.coli) 乙醇脱氢酶[大肠杆菌] 1000 E-ADHEC
Alpha-Amylase(A.oryzae) Alpha淀粉酶[米麴菌] 20000 E-ANAAM
Alpha-Amylase(B.licheniformis) Alpha淀粉酶[苔藓杆菌] 120000 E-BLAM
β-Amylase(Barley) Beta淀粉酶[大麦] 100000 E-BARBL
β-Amylase(Barley) Beta淀粉酶[大麦] 2g E-BARBP
β-Amylase(B.cereus) Beta淀粉酶[仙人掌杆菌] 100,000 E-BCBAM
Amyloglucosidase(A.niger) 淀粉葡萄糖苷酶[黑曲霉] 140,000 E-AMGDF
Amyloglucosidase(Rhizopus sp.) 淀粉葡萄糖苷酶[根霉] 5,000 E-AMGPU
endo-1,5-α-L-Arabinanase(A.niger) 1,5-α-L-阿拉伯糖内切酶[黑曲霉] 140 E-EARAB
α-L-Arabinofuranosidase(A.niger)) α-L-阿拉伯呋喃糖酶[黑曲霉] 300 E-AFASE
Recα-L-Arabinofuranosidase(Novel Specificity) α-L-阿拉伯呋喃糖酶[新] 400 E-AFAM2
Alpha-aspartyl dipeptiease(E.coli) Alpha-天冬氨酰二肽酶[大肠杆菌] 2,000 E-DIPEP
Celobiohydrolase I(T.longibrachiatum) 纤维素二糖水解酶I[长枝木霉] 20mg E-CBHI
Cellulase(endo-1,4-D-Glucanase)(T.longibrachiatum) 纤维素酶[1,4-D-葡聚糖内切酶[长枝木霉] 1,000 E-CELTR
Cellulase(endo-1,4-D-Glucanase)(T.emersonii) 纤维素酶[1,4-D-葡聚糖内切酶[Emersonii蓝状菌] 2,000 E-CELTE
Celluase(endo-1,4-D-glucanse)(A.niger) 纤维素酶[1,4-D-葡聚糖内切酶[黑曲霉] 2,000 E-CELAN
Reccitrate synthase(E.coli) 柠檬酸合酶[大肠杆菌] 50 E-CITEC
Cytidylate Kinase(Prokaryote) 胞嘧啶核苷酸激酶[原核生物] 500 E-CMPK
RecDiaphorase(D.coli) 黄递酶[大肠杆菌] 1,000 E-DIAEC
Formate dehydrogenase(C.boidini) 甲酸脱氢酶[C.boidini] 300 E-FDHCB
CructanaseMixture(Purified) 果糖酶混合物[已纯化] 40ml E-FRMXLQ
Fructanase Mixture Powder 果糖酶混合物 40,000 E-FRMXPD
endo-1,4-β-D-Galactanase(A.niger) 1,4-β-D-半乳糖内切酶[黑曲霉] 1,000 E-EGALN
RecGalactose Dehydrogenase(soli prokaryote) 半乳糖脱氢酶[土壤原核生物] 200 E-GALDH
α-Galactosidase(A.niger) α半乳糖苷酶[黑曲霉] 2,000 E-AGKAN
α-Galactosidase(G.uar) α半乳糖苷酶[瓜尔豆] 600 E-AGLGU
β-Galactosidase(A.niger) β半乳糖苷酶[黑曲霉] 8,000 E-BGLAN
β-Galactosidase(Lactase)(K.fragilis) β半乳糖苷酶[乳糖酶][脆壁克鲁维酵母] 4,000 E-LACTS
endo-1,3-β-Glucanase(Trichoderma.sp.) 1,3-β-D葡聚糖内切酶[木霉菌] 100 E-LAMSE
exo-1,3-β-Glucanase(Trichoderma.sp.) 1,3-D-β-葡聚糖外切酶[木霉菌] 400 E-EXBGL
Gluconokinase(E.coli) 葡糖酸激酶[大肠杆菌] 1,500 E-GLUKEC
Glucose 6-phosphate dehydrogenase 葡萄糖6磷酸脱氢酶 5,000 E-GPDH5
Glucose 6-phosphate dehydrogenase 葡萄糖6磷酸脱氢酶 25,000 E-GPDH25
a-Glucosidase(Maltase)(yeast) Alpha葡[萄]糖苷酶[麦芽糖酶][酵母] 2,000 E-MALTS
a-Glucosidase(B.stearothermophilus) Alpha葡[萄]糖苷酶[嗜热脂肪芽孢杆菌] 1,500 E-TSAGL
a-Glucosidase(Transglucosidase)(A.niger) Alpha葡[萄]糖苷酶[转葡萄糖苷酶][黑曲霉] 2,000 E-TRNGL
β-Glucosidase(A.niger) Beta葡[萄]糖苷酶[黑曲霉] 200 E-BGLUC
Recβ-Glucosidase(Agrobacterium sp.) Beta葡[萄]糖苷酶[农杆菌] 600 E-BGISAG
Recβ-Glucosidase(thermostable)(T.maritima) Beta葡[萄]糖苷酶[耐热][热海栖热孢菌] 230 E-BGOSTM
Glutamate dehydrogenase(E.coli) 谷氨酸脱氢酶[大肠杆菌] 10,000 E-GLDHEC
RecGlutamate Oxaloccetate Transaminase(E.coli) 谷氨酸草酰乙酸转氨酶[大肠杆菌] 5,000 E-GOTEC
Glutamate pyruvate transminase(B.subtilils) 谷氨酸丙酮酸转氨酶[枯草杆菌] 2,500 E-GPTBS
Glucose Oxidase/catalase Mixture 葡萄糖氧化酶/催化酶混合物 2 vials E-GOXCA
Guanylate Kinase(prokayote) 鸟苷酸激酶[原核生物] 500 E-GMPK
3-Hydrxybutyrate dehydrogenase(prokaryote) 3-羟丁酸脱氢酶[原核生物] 200 E-HBDH
Hexokinase 已糖激酶 10,000 E-HEX10
Hexokinase 已糖激酶 50,000 E-HEX50
Hexokinase(420U/ml)+G6PDH(210U/ml) 已糖激酶[420U/ml]+葡萄糖-6-磷酸脱氢酶[210U/ml] 10ml E-HKGDH
RecHaluronate lyase(novel specificity)(soil prokaryote) 透明质酸[裂解]酶[新品][土壤原核生物] 50 E-HYLSP
endo-Inulinase(A.niger) 菊粉内切酶[黑曲霉] 200 E-ENDOI
exo-Inulinase(A.niger) 菊粉外切酶[黑曲霉] 5000 E-EXOI
Invertase(Fructofuranosidase)(yeast) 蔗糖酶[呋喃果糖苷酶][酵母] 150000 E-INVRT
Isoamylase(Glycogen 6-glucanohydrolase) 异淀粉酶[糖原6-葡萄糖苷酶] 500 E-ISMY
Isocitrate dehydrogenase (B.subtilis) 异柠檬酸脱氢酶[枯草杆菌] 2000 E-ICDHBS
Lichenase(endo-1,3(4)-D-Glucanase)(Bacillussp.) 地衣聚糖酶[1,3(4)-D-葡聚糖内切酶[芽孢杆菌] 5000 E-LICHN
RecD-Malate dehydrogenase(E.coli) D-苹果酸脱氢酶[大肠杆菌] 200 E-DMDHEC
RecL-Malate dehydrogenase(E.coli) L-苹果酸脱氢酶[大肠杆菌] 50000 E-LMDHEC
endo-1,4-β-Mannanase(A.niger)) 1,4-β甘露聚糖内切酶[黑曲霉] 500 E-BMANN
endo-1,4-β-Mannanase(Bacillus sp.) 1,4-β甘露聚糖内切酶[芽孢杆菌] 2000 E-BMABS
RecMannitol dehydrogenase(P.fluorescens) 甘露醇脱氢酶[荧光假单胞菌] 500 E-MNHPF
Rec-β-Mannosidase(C.fimi) β甘露糖苷酶[纤维单胞菌] 200 E-BMOSCF
Pectte Lyase M1(Aspergillus sp.) 果胶酸裂合酶M1[曲霉属] 600 E-PECLY
RecPectate Lyase M2(C.japonicus) 果胶酸裂合酶M2[日本龟蜡蚧] 2500 E-PLYCJ
RecPhosphoglucose Isomerse (B.subtillis) 磷酸葡糖异构酶[枯草杆菌] 5000 E-PGIBS
RecPhosphoglucose Isomerse (B.subtillis) 磷酸葡糖异构酶[枯草杆菌] 50000 E-PGIBSB
RecPhosphoglucose Isomerse (E.coli) 磷酸葡糖异构酶[大肠杆菌] 10000 E-PGIEC
RecPhosphoglucose Isomerse (E.coli) 磷酸葡糖异构酶[大肠杆菌] 50000 E-PGIECB
6-Phosphogluconate dehydrogenase(E.coli) 6-磷酸葡糖酸脱氢酶[大肠杆菌] 150 E-PGDHEC
RecPhosphoglucose Isomerse (S.cerevisiae) 磷酸葡糖异构酶[酿酒酵母] 5000 E-PGISC
RecPhosphoglucose Isomerse (S.cerevisiae) 磷酸葡糖异构酶[酿酒酵母] 50000 E-PGISCB
RecPhosphoglucose Isomerse (E.coli) 磷酸甘露糖异构酶[大肠杆菌] 1000 E-PMIEC
endo-Polygalacturonanase M1(A.niger) 聚半乳糖醛酸M1[黑曲霉] 5000 E-PGALS
endo-Polygalacturonanase M2(A.niger) 聚半乳糖醛酸M2[黑曲霉] 10000 E-PGALUSP
Protease(Subtillisin A)(B.licheniformis) 蛋白酶[枯草杆菌][对地衣芽孢杆菌] 2 g(40ml) E-BSPRT
Pullulanase M1(K.planticola) 普鲁兰酶M1[植生克雷伯氏菌] 2000 E-PULKP
Pullulanase M2(B.licheniformis) 普鲁兰酶M2[对地衣芽孢杆菌] 2000 E-PULBL
Pullulanase M3(B.acidopullulyticus) 普鲁兰酶M3[嗜酸普鲁兰芽孢杆菌] 5000 E-PULBA
Succinyl-CoA synthetase(prokaryote) 琥珀酰辅酶A合成酶[原核生物] 550 E-SCOAAS
Sucrase (Maltase)(yeast) 蔗糖酶[麦芽糖酶][酵母] 220 E-SUCR
Thymidylate Kinase (prokaryote) 胸苷酸激酶[原核生物] 150 E-TMPK
Xylanase M1(T.viride) 木聚糖酶M1[绿色木霉菌] 8000 E-XYTR1
Xylanase M2(T.longibrachiatum;pl 5.5) 木聚糖酶M2[长枝木霉] 4000 E-XYTR2
Xylanase M3(T.longibrachiatum;pl 9.0) 木聚糖酶M3[长枝木霉] 8000 E-XYTR3
Xylanase M4(A.niger) 木聚糖酶M4[黑曲霉] 8000 E-XYTR4
Xylanase M6(rumen microorganism) 木聚糖酶M6[瘤胃微生物] 8000 E-XYRU6
Alkaline phosphatase(E.coli) 碱性磷酸酶(大肠杆菌) 400 E-ALPEC
Glutaminase(E.coli) 谷氨酰胺酶(大肠杆菌) 2500 E-GLUTEC
Guanylate Kinase(prokayote) 鸟[嘌呤核]苷酸激酶(原核生物) 500 E-GMPK
3-Hydrxybutyrate dehydrogenase(prokaryote) 3-羟基苯甲酸酯脱氢酶(原核生物) 200 E-HBDH
myo-Inositol dehydrogenase(B.subtillis) 肌环己六氢醇脱氢酶(枯草芽孢杆菌) 500 E-INDH
D-Lactate dehydrogenase(L.mesenteroides) D-乳酸盐脱氢酶(肠膜明串珠菌) 15000 E-DLDHLM
β-Mannanase(T.maritima) Beta甘露聚糖酶(海栖热袍菌) 250 E-BMATM
Malt Amylase Standard 麦芽淀粉酶标准液 100ml E-MAST

 

蔗糖/D-果糖/D-葡萄糖检测试剂盒

蔗糖/D-果糖/D-葡萄糖检测试剂盒 Sucrose/Fructose/D-Glucose Assay Kit

产品名称:蔗糖/D-果糖/D-葡萄糖检测试剂盒

英文名称:Sucrose,D-fructose and D-glucose sucrose assay kit

货号:K-SUFRG

型号规格:150 (50 of each)

优点:价格低廉(每次检测成本);

所有试剂配制后的稳定性〉12个月;

反应快;葡萄糖氧化酶/过氧化酶/己糖激酶/6-磷酸葡萄糖脱氢酶;

包含标准品

检测原理:


检测方法:紫外分光光度法

检测波长:340nm;

反应时间:30min;

检测限:1.38mg/L;

适用样品:啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、面包、焙烤产品、乳制品、糖果、甜点、糖食、冰淇淋、水果和蔬菜(如:马铃薯)、肉制品(如香肠)、调味品、饲料、烟、化妆品、纸及其他原料(如生物培养、样品等);

方法认证:EN。NEN。NF。DIN,GOST,IFU ,AIJN,MEI,IOCCC以及德国、奥地利认证

商品说明:
The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials

Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC
Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Stabilised D-glucose / D-fructose standard solution included
  • Extended cofactors stability

甘油检测试剂盒 Glycerol GK Assay Kit

甘油检测试剂盒 Glycerol GK Assay Kit
产品货号:K-GCROL
产品品名: 甘油检测试剂盒
英文品名 :Glycerol Assay Kit
规格型号: 70 assays per kit
甘油是食品加工业中通常使用的甜味剂和保湿剂,大多出现在运动食品和代乳品中。
上海甄准生物提供 甘油检测试剂盒 ,紫外吸光度法( 340nm )测定食品、饮料及其他原料中的甘油。
原理:
反应时间: 5min
检测限: 0.37mg/L
适用样品: 葡萄酒(葡萄汁)、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸(硬纸板)、化妆品
优点:
l    新型的片剂形式增加了稳定性
l    价格低廉(每次检测成本)
l    所有试剂配制后的稳定性 >2 年
l    反应快
l    该方法已通过 OIV , MEBAK 以及德国和瑞士的认证

商品说明:
The Glycerol GK test kit is a simple, reliable and accurate method for the measurement and analysis of glycerol in beverages, foodstuffs and other material. Based on use of ADP-glucokinase and increase in absorbance on conversion of NAD+ to NADH.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(ADP-GK)
(2) ADP + D-glucose → G-6-P + AMP
(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NAD+ → gluconate-6-phosphate + NADH + H+
Kit size: 70 assays (manual) / 700 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 7 min
Detection limit: 0.37 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method
Advantages

  • Novel tablet format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Positive reaction (assay proceeds with an increase in absorbance)
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

纤维素酶检测试剂盒

纤维素酶检测试剂盒[CellG5法], Cellulase Assay Kit (CellG5 Method)

货号:K-CellG5
中文品名:纤维素酶检测试剂盒[CellG5法]
品名:Cellulase Assay Kit (CellG5 Method)
规格1:K-CellG5-4V 120/240次(手工)/480次(自动分析仪)
规格2:K-CellG5-2V 60/120次(手工)/240次(自动分析仪)
说明:CellG5检测试剂盒用于检测纤维素内切酶(1,4 -β-葡聚糖内切酶)包含两种组分:1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2)热稳定的β-葡萄糖苷酶。 酮阻断基团可阻止β-葡萄糖苷酶对BPNPG5的水解。通过纤维素内切酶的培养可生成非阻断的显色寡糖,被β-葡萄糖苷酶迅速水解。产生4-硝基苯酚的速率与β-葡萄糖苷酶水解BPNPG5直接相关。加入Tris缓冲液(pH 9.0)后可终止反应,并形成苯酚显色产物。
CellG5检测法代表了代表了纤维素酶测量方法的巨大进步。传统的纤维素酶检测依赖于酶底物,例如羧甲基纤维素,微晶纤维素,纤维低聚糖,CMC滤纸,染色多糖,包括CMC刚果红或纤维素天青。
应用:纤维素酶检测试剂盒[CellG5法]。

原理:
比色法测定酶制剂、发酵产品中1,4 -β-葡聚糖内切酶(纤维素酶)的含量。
纤维素酶检测试剂盒 (K-CellG5)检测原理
检测方法:分光光度法 @400 nm
反应时间:10min
检测限:3.5 x 10-4 U/mL
应用案例:
发酵液,工业酶制剂和生物燃料研究。
方法识别:
新方法
试剂盒组成:
Bottle 1: (x2) 或 (x4) 每瓶含有4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5)溶于3毫升含有0.02% w/v叠氮钠的10% DMSO水溶液。
-20°C下稳定超过4年。
Bottle 2: (x 2) 热稳定的β-葡萄糖苷酶(0.50 mL, 600 U/mL)溶于含有0.02% w/v叠氮钠的 50% w/v 硫酸铵溶液。
4°C下稳定超过4年。
Bottle 3: 木霉纤维素酶标准溶液 (5 mL, ~ 3 U/mL; 准确至见瓶标签) 溶于含有0.02% w/v叠氮钠的50% 的丙三醇水溶液。
-20°C下稳定超过4年。
优点:

所有试剂可稳定超过4年
价格非常具有竞争力
完全针对纤维素酶(1,4-葡聚糖内切酶)
常规操作,非常灵敏
检测方法十分简单,适合仪器分析
包含标准
参考文献:
Novel substrates for the measurement of endo-1,4-β-glucanase (endo-cellulase). McCleary, B. V., Mangan, D., Daly, R., Fort, S., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 385, 9-17.
Quantitative fluorometric assay for the measurement of endo-1,4-β-glucanase. Mangan, D., McCleary, B. V., Liadova, A., Ivory, R. & McCormack, N. (2014). Carbohydrate Research, 395, 47-51.
A novel automatable enzyme-coupled colorimetric assay for endo-1,4-β-glucanase (cellulase). Mangan, D., Cornaggia, C., McKie, V., Kargelis. T. & V. McCleary, B. V. (2016). Analytical and Bioanalytical Chemistry, 408(15), 4159-4168.
说明书下载地址:https://secure.megazyme.com/files/Booklet/K-CellG5_DATA.pdf

内切纤维素酶检测试剂盒

内切纤维素酶检测试剂盒

英文名:Cellulase Assay Kit (CELLG3 Method)
货号:K-CELLG3
规格:180 / 360 assays per kit / 720 (auto-analyser)
市场价: 5537

 纤维素酶是一种重要的酶产品,是一种复合酶,主要由外切β-葡聚糖酶、内切β-葡聚糖酶和β-葡萄糖苷酶等组成,还有很高活力的木聚糖酶。
提供内切纤维素酶检测试剂盒,色度法(400 nm)测定酶制品和发酵产品中的纤维素酶(内切-1,4-β-葡聚糖酶)。
内切纤维素酶检测试剂盒(CELLG3方法) K-CELLG3
使用高纯度β-葡萄糖苷酶和苯亚甲基阻断,2 – 氯-4 – 硝基苯基-β-Dcellotrioside(BClPNPβ-G3)。 β-葡萄糖苷酶的能力确保测定的可靠的最大的灵敏度。BClPNPβ-G3的水解为苯亚甲基,通过纤维素酶阻断纤维二糖和2-氯-4 -硝基苯基-β-D-葡萄糖, 2-Cl-4-硝基苯基-β-D-葡萄糖通过β-葡萄糖苷酶立即裂解为D-葡萄糖和游离的2 – 氯-4 – 硝基苯酚(ClPNP)。
反应时间:16min
适用样品:酶制品和发酵产品
优点:
价格低廉(每次检测成本)
特异性高
方法简单
包含标准品
 
The CELLG3 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components;

1) 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-D-cellotrioside (BCNPG3) and 2) thermostable β-glucosidase. The benzylidene blocking group prevents any hydrolytic action by the β-glucosidase on BCNPG3.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase.  The rate of formation of 2-chloro-4-nitrophenol is therefore directly related to the hydrolysis of BCNPG3 by the endo-cellulase.  The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
Please note that a new assay kit (K-CELLG5) is now available for the measurement of endo-cellulase.  The CELLG5 reagent contains a cellopentaose core and exhibits vastly improved sensitivity for some cellulases.  In addition, the exchange of the benzylidene blocking group in CELLG3 for 3-keto-butylidene in CELLG5 improves the substrate’s water solubility significantly, allowing for a reduction in the concentration of DMSO required in the assay.  As DMSO is known to inhibit certain cellulases, this is another benefit in using CELLG5.  Megazyme now recommends the use of K-CELLG5 for all assays for the measurement of endo-cellulase.
Colourimetric method for the determination of 
endo
-1,4-β-glucanase (cellulase) in enzyme preparations and fermentationproducts
Principle:
(endo-1,4-β-glucanase)
(1) 3-Ketobutylidene-G5-β-PNP + H2O → Blocked-GX + G(5-X)-β-PNP
(thermostable β-glucosidase)
(2) G(5-X)-β-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-CELLG5-4V 120 / 240 assays (manual) / 480 (auto-analyser)
or
K-CELLG5-2V 60 / 120 assays (manual) / 240 (auto-analyser)
Method:                         Spectrophotometric at 400 nm
Total assay time:           10 min
Detection limit:                3.5 x 10-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, biofuels research
Method recognition:     Novel method

 

Beta葡聚糖[酵母和蘑菇]检测试剂盒

Beta葡聚糖[酵母和蘑菇]检测试剂盒 β-Glucan Assay Kit (Yeast & Mushroom)

产品名称:Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称:Mushroom and yeast beta-glucan assay Kit

货号: K-YBGL

型号规格:100次

优点:成本低;所有试剂配制后的稳定性〉2年;只有酶试剂盒可用;;方法简单;包含标准品

检测原理:

检测方法:比色法

检测波长:510nm;

总检测时间:100min;

检测限:样品重量的1-100%;

适用样品:酵母制备、蘑菇制备及其他原料;

方法认证:新方法

商品说明:
For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit
Colourimetric method for the determination of Yeast and 
Mushroom β-Glucan in yeast, mushroom, foodstuffs and 
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → 
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)
(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose
(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
 quinoneimine + 4H2O
(amyloglucosidase)
(6) α-Glucan + H2O    →    D-glucose
Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method
Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

甘油检测试剂盒

 

甘油检测试剂盒 Glycerol Assay Kit
产品货号:K-GCROL
产品品名: 甘油检测试剂盒
英文品名 :Glycerol Assay Kit
规格型号: 70 assays per kit
甘油是食品加工业中通常使用的甜味剂和保湿剂,大多出现在运动食品和代乳品中。
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

原理: (glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate
(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+
反应时间: 5min
检测限: 0.37mg/L
适用样品: 葡萄酒(葡萄汁)、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸(硬纸板)、化妆品
优点:
l    新型的片剂形式增加了稳定性
l    价格低廉(每次检测成本)
l    所有试剂配制后的稳定性 >2 年
l    反应快
l    该方法已通过 OIV , MEBAK 以及德国和瑞士的认证
REFERENCES:

  1.         Spinella, C. I. (1966). Modified enzymatic procedure for the routine determination of glycerol  and triglycerides in plasma.  J. Lipid Res ., 7,167-169 .
  2.  Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and  glycerol in beer.  J. Inst. Brew ., 92, 225- 228.
  3.         Michal, G. (1976). Enzymatische analyse in der pharmazie.  Acta Pharmaceutica  Technologica , Suppl. 1, S, 151-162.
  4.         Pfandl, A. & Menschig, D. (1984). Ein beitrag zur enzymatischen glycerin- und  ethanol-bestimmung.  Pharm. Ind ., 46, 403-407.
  5.         Wieland, O. H. (1988). Glycerol. In  Methods of Enzymatic Analysis  (Bergmeyer, H. U., ed.),  3rd ed., Vol. VI, pp. 504-510, VCH Publishers (UK) Ltd., Cambridge, UK.

直链淀粉/支链淀粉[胶淀粉]检测试剂盒

直链淀粉/支链淀粉[胶淀粉]检测试剂盒

名称:直链淀粉/支链淀粉(胶淀粉)检测试剂盒
产品货号:K-AMYL
产品品名:直链/支链淀粉检测试剂盒
英文品名:Amylose/Amylopectin assay Kit
规格型号:100 assays per kit
规格: 100次
产地:爱尔兰
用途:用于测量谷物和面粉中直链淀粉/支链淀粉的比例和淀粉含量。
标配:四玻璃瓶加两个塑料瓶
在水中的溶解度:易溶
pH值:中性
气味:无
状态:粉末和液体
保持期:10年以上(冰箱内)

      谷类淀粉的许多特性决定其最终用途,这些取决于直链淀粉/支链淀粉的比率。这些特性包括糊化和凝胶化,溶解度,抗性淀粉的形成以及整颗大米的烹饪和构造特性。因此,淀粉中直链淀粉含量的测定是淀粉加工的一个重要的质量参数。

      最常用的测定谷物淀粉中直链淀粉含量的方法是利用电势,电流测定或直链淀粉的碘结合能力比色测定直链淀粉-碘色合配合物。然而,这些方法具有不确定性。支链淀粉-碘复合物也可以形成,这样降低了利用非比色法测定的游离碘离子的浓度,并且用比色法测量时,该复合物可能和直链淀粉-碘复合物吸收相同波长的光。这种复合物致使直链淀粉的测定含量超过实际含量,需要进行校正。Gibson等详细列举了使用这些方法所遇到的许多其他问题。 支链淀粉结合ConA的特殊复合物为淀粉中直链淀粉的测定提供了一种替代方法,而且不存在不确定性问题。在指定pH值,温度和离子强度的条件下,ConA特异性结合分支多糖并形成沉淀,这种结合以多个非还原性末端基团上的α-D-吡喃葡萄糖基或α-D-吡喃甘露糖基单位为基础。因此,ConA可以有效结合淀粉中的支链淀粉成分,但是不能结合线性为主的直链淀粉成分。

前言:
谷类淀粉的许多特性决定其最终用途,这些取决于直链淀粉/支链淀粉的比率。这些特性包括糊化和凝胶化,溶解度,抗性淀粉的形成以及整颗大米的烹饪和构造特性。因此,淀粉中直链淀粉含量的测定是淀粉加工的一个重要的质量参数。
最常用的测定谷物淀粉中直链淀粉含量的方法是利用电势,电流测定或直链淀粉的碘结合能力比色测定直链淀粉-碘色合配合物。然而,这些方法具有不确定性。支链淀粉-碘复合物也可以形成,这样降低了利用非比色法测定的游离碘离子的浓度,并且用比色法测量时,该复合物可能和直链淀粉-碘复合物吸收相同波长的光。这种复合物致使直链淀粉的测定含量超过实际含量,需要进行校正。Gibson等详细列举了使用这些方法所遇到的许多其他问题。
支链淀粉结合ConA的特殊复合物为淀粉中直链淀粉的测定提供了一种替代方法,而且不存在不确定性问题。在指定pH值,温度和离子强度的条件下,ConA特异性结合分支多糖并形成沉淀,这种结合以多个非还原性末端基团上的α-D-吡喃葡萄糖基或α-D-吡喃甘露糖基单位为基础。因此,ConA可以有效结合淀粉中的支链淀粉成分,但是不能结合线性为主的直链淀粉成分。
此方法是Yun和Matheson改进的ConA方法。分析之前用乙醇预处理去除脂质。
直链淀粉/支链淀粉[胶淀粉]检测试剂盒  原理:
淀粉样品通过加热完全地溶解在二甲基亚砜(DMSO)里。用乙醇沉淀淀粉去除其中的脂质,回收沉淀的淀粉。用醋酸/盐溶液溶解沉淀的样品,加入ConA,特异性沉淀支链淀粉,离心去除沉淀。单位体积上清液中的直链淀粉用酶水解为D-葡萄糖,然后用葡萄糖氧化酶/过氧化物酶试剂进行测定。另外一份单位体积醋酸/盐溶液中的总淀粉同样用酶水解为D-葡萄糖,然后加入葡萄糖氧化酶/过氧化物酶,用比色法测定。
根据ConA沉淀样品的上清液和与总淀粉样品中的GOPOD在510 nm处的吸光光度值之比判断直链淀粉在总淀粉中的含量。
该方法适用于所有的纯淀粉和谷物粉。
精确性
样品如为纯淀粉,相对标准偏差为<5%。
样品如为谷物面粉,相对标准偏差为~10%。
Amylose/Amylopectin Kits
For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.
Content: 100 assays per kit
Appearance Four glass vials plus two plastic vials
Specific Gravity Not applicable
Solubility in Water Most components readily soluble.
pH Value neutral
Odour none
Form Powders and liquid.
Stability stable in a refrigerator for ten or more years
Ingredients
Name Proportion
Concanavelin A 1 vial (glass)
Amyloglucosidase/a-Amylase 1 vial (glass)
Glucose Buffer 1 vial (polypropylene)
Glucose Assay reagent 1 vial (glass)
Glucose standard and Starch reference 1 vial each (glass and plastic)

直链淀粉/支链淀粉(胶淀粉)检测试剂盒组成成分:

瓶子1:冻干的Con A (伴刀豆球蛋白 A concanavalin A) ,200 mg,-20℃下稳定性>5年。(玻璃)

瓶子2:淀粉葡糖苷酶(淀粉葡萄糖苷酶/α-淀粉酶)【200U,条件为消化对硝基苯基β-麦芽糖苷(也就是3300U,条件为pH4.5,40℃下消化淀粉)】加上真菌α-淀粉酶(500U ,条件为pH5.0,40℃下消化 Ceralpha 试剂),2mL,4℃下稳定性>5年。   (玻璃)

瓶子3:GOPOD 试剂缓冲液(葡萄糖缓冲液)。磷酸钾缓冲液(1M,pH7.4),对羟苯甲酸(0.22M)和叠氮化钠(0.02% W/W)。4℃下稳定性> 3年。(聚丙烯)

瓶子4:GOPOD试剂酶(葡萄糖检测试剂 )。葡糖氧化酶(>12,000U)加上过氧化物酶(>650U)和4-氨基安替比林(80mg)。冻干粉,-20℃下稳定性>5年。

瓶子5:D-葡萄糖标准溶液(5 mL,1.0mg/mL)溶于苯甲酸0.2%(w/v)。室温下稳定性>5年。(玻璃)

瓶子6:淀粉参考样品(含有特定含量的直链淀粉),室温下稳定性>5年。(塑料)

试剂制备的准备:

1、用50mlCon A试剂将瓶子1中药品溶解,分成适当的几部分存放在聚丙烯管中,尽可能在-20℃下使用或冷藏。-20℃下稳定性>2年。

2、用20ml醋酸钠缓冲液将瓶子2中的药品溶解,分成适当的几部分存放在聚丙烯管中,

3、用蒸馏水将中的药品(GOPOD 试剂缓冲液)稀释至1L。现配现用。

(注意:如果瓶子3意外存放在-20℃下,一些盐可能会结晶。配制时要确保所有的结晶物质都溶解在1L的蒸馏水中)

4、用20ml GOPOD 试剂缓冲液溶解GOPOD试剂酶,并定量的转移到存放GOPOD 试剂缓冲液的瓶子中,用铝箔封住瓶子,避光保存。这种试剂黑暗中保存,2-5℃下能保存大约3个月,-20℃下稳定性大于12个月。

5、按提供的方法使用D-葡萄糖标准溶液和淀粉参考样品,室温下稳定性>5年。

安全考虑:

1、DMSO对皮肤有刺激性,用的时候要格外小心。它通过皮肤被吸收,对皮肤和眼睛都有刺激性。穿保护装、戴手套,避免溶剂溅出。尽可能在通风橱中进行试验。

2、ConA通过呼吸、皮肤接触或摄入体内,对人体是十分有害的。这种伤害不可逆转,可能会致畸。在使用ConA结晶体球蛋白和含有ConA的溶剂时要穿保护装、戴手套和特制的面具。

3、叠氮化钠是有毒的化学试剂,必须要有专门的处理。它要添加特殊的缓冲液来充当防腐剂。毒性通过缓冲可以被消除,但是缓冲液必须在4℃下保存。

缓冲液或溶剂:

1、乙酸钠缓冲液(10mM,pH 4.5)

加5.9ml1.05g/ml的冰醋酸到900ml的蒸馏水中用4g/100ml的NaOH溶液调pH到4.5(大约30ml),加0.2g叠氮化钠,用蒸馏水定容至1L。室温下稳定性>2年。

2、ConA 浓缩液(600 mM, pH 6.4 乙酸钠缓冲液)

称取49.2g无水醋酸钠,175.5g氯化钠,0.5gCaCl2.2H2O ,0.7 gMgCl2.6H2O 和0.7 g MnCl2.4H2O ,用900 mL 蒸馏水将其溶解逐滴加入冰醋酸调pH为6.4,再用蒸馏水定容至1L。4℃下保存2周。(注意:配制这种复合缓冲液时,pH的调节特别重要,如果pH低于

6.4,就会有沉淀物形成,就算pH再调回来,沉淀物也不会溶解。结果会导致缓冲液必须丢弃重新配制。)

3、ConA 溶液(使用浓度)

将30ml ConA 浓缩液用蒸馏水稀释至100ml。现配现用。

4、DMSO

分析纯试剂。室温下可保存5年。

预处理:淀粉样品事先用乙醇进行预处理,除去脂质。如果样品没有用乙醇进行预处理,在某些样品中测得的直链淀粉含量会低50%。

实验步骤:

A、淀粉预处理

1、准确称取淀粉或面粉样品至10ml的样品管中。记录样品重量精确到0.1mg。

2、加1ml DMSO至试管,在漩涡混合器中慢速混匀,在沸水浴中加热直至分散(大约1分钟)确保淀粉没有结团结块。

3、密封试管,高速混匀。沸水浴加热15min,再高速间歇混匀。

4、室温放置大约5min,加2ml 95%乙醇,混匀,再加4ml乙醇,盖上盖子,颠倒混匀。形成淀粉沉淀物,将试管静止15min(条件允许过夜静止)。

5、2000r,5min离心,弃去上清液,倒置在纸巾上10min,确保乙醇全部挥发。在接下来直链淀粉和淀粉的测定中使用此沉淀物。

6、加2ml DMSO到淀粉沉淀物中,将试管沸水浴15min(间或混匀),确保没有块状。

7、从沸水浴中取出试管,立即加入4ml ConA 溶液,将试管中药品转移至25ml容量瓶中。用 ConA 溶液定容。

(整个过程必须在2h内完成)

B、支链淀粉- ConA 沉淀物和直链淀粉的测定

1、量取1mlA试剂到2ml的试管中,加0.54ml的ConA 溶液,盖上盖子,反复颠倒,混匀。避免样品起泡。

2、室温静置1h。14000r,10min,室温下离心。

3、转移1ml的上清液到15ml的离心管中,,加3ml100mM醋酸钠缓冲液,pH4.5,混合药品。轻轻塞住管口,沸水浴5min,使ConA变性。

4、将试管在40℃下水浴,平衡5min,,加1ml淀粉转葡萄糖苷酶和a-淀粉酶的混合物,40摄氏度下反应30min,2000r离心5min。

5、准确量取1ml上清液,加4ml GOPOD试剂,40摄氏度下反应20min,使空白试剂和D-葡萄糖标准液同时反应。

6、在510nm下测定每一个样品和D-葡萄糖标准液的吸光光度值。

C、总淀粉的测定

1、将0.5mlA溶液和4ml100mM乙酸钠溶液混合,pH 4.5

2、加1ml淀粉转葡萄糖苷酶和a-淀粉酶的混合物,40摄氏度下反应10min,

3、准确量取1ml上清液,加4ml GOPOD试剂,40摄氏度下反应20min。这个反应必须让样品和标准液按B部分同时进行。

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淀粉损失检测试剂盒

淀粉损失检测试剂盒,Starch Damage Assay Kit

英文名:Starch Damage Assay Kit
中文品名:淀粉损伤检测试剂盒
货号:K-SDAM
规格:200 assays per kit,200 次检测
应用:淀粉损伤检测试剂盒,用于谷物面粉中淀粉损伤的检测和分析。

原理:
谷物淀粉中淀粉损伤紫外检测方法
淀粉损伤检测试剂盒 (K-SDAM)检测原理
检测方法:分光光度法 @510 nm
反应时间:~ 40分钟
检测限:样品重量的0.5-100%
应用案例:
谷物面粉和其它食材。
方法识别:
符合AACC (方法 76-31.01), ICC (标准号 164) 和 RACI (标准方法)。
试剂盒组成:
Bottle 1: 真菌α-淀粉酶(pH5.4和40°C条件下,10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸铵悬浮液。
4°C下稳定超过 3年
Bottle 2: 淀粉葡萄糖苷酶(pH 4.5 和40°C条件下,4 mL, 200 U/mL 在可溶性淀粉中)。硫酸铵悬浮液。
4°C稳定超过3年
Bottle 3: GOPOD 试剂缓冲液。缓冲液(50 mL, pH7.4), 对羟基苯甲酸和叠氮钠(0.095% w/v).
4°C稳定超过4年
Bottle 4: GOPOD 试剂酶。葡萄糖氧化酶,过氧化酶和4-氨基安替比林。冻干粉。
-20°C稳定超过5年
Bottle 5: D-葡萄糖标准溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
室温下稳定超过5年
Bottle 6: 小麦面粉标样。淀粉损伤程度在小瓶标签上注明。
室温下稳定超过5年

Megazyme 淀粉损伤检测试剂盒(K-SDAM)

淀粉损伤检测试剂盒, Starch Damage Assay Kit , 货号:K-SDAM
品牌:Megazyme
货号:K-SDAM
中文品名:淀粉损伤检测试剂盒
品名:Starch Damage Assay Kit
规格:200 次检测
应用:淀粉损伤检测试剂盒,用于谷物面粉中淀粉损伤的检测和分析。
原理:
谷物淀粉中淀粉损伤紫外检测方法
淀粉损伤检测试剂盒 (K-SDAM)检测原理
检测方法:分光光度法 @510 nm
反应时间:~ 40分钟
检测限:样品重量的0.5-100%
应用案例:
谷物面粉和其它食材。
方法识别:
符合AACC (方法 76-31.01), ICC (标准号 164) 和 RACI (标准方法)。
试剂盒组成:
Bottle 1: 真菌α-淀粉酶(pH5.4和40°C条件下,10 mL, 1,000 U/mL on Ceralpha reagent* )。硫酸铵悬浮液。
4°C下稳定超过 3年
Bottle 2: 淀粉葡萄糖苷酶(pH 4.5 和40°C条件下,4 mL, 200 U/mL 在可溶性淀粉中)。硫酸铵悬浮液。
4°C稳定超过3年
Bottle 3: GOPOD 试剂缓冲液。缓冲液(50 mL, pH7.4), 对羟基苯甲酸和叠氮钠(0.095% w/v).
4°C稳定超过4年
Bottle 4: GOPOD 试剂酶。葡萄糖氧化酶,过氧化酶和4-氨基安替比林。冻干粉。
-20°C稳定超过5年
Bottle 5: D-葡萄糖标准溶液(5 mL, 1.5 mg/mL) 溶于0.2% (w/v)苯甲酸。
室温下稳定超过5年
Bottle 6: 小麦面粉标样。淀粉损伤程度在小瓶标签上注明。
室温下稳定超过5年
优点:

价格非常具有竞争力
所有试剂制备后可以稳定保存超过2年
仅提供酶法检测试剂盒
特异性
操作简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准品
The Starch Damage test kit is suitable for the measurement and analysis of starch damage in cereal flours.
Colourimetric method for the determination of Starch Damage
in cereal flours

Principle:
(fungal α-amylase)
(1) Damaged (or gelatinised) starch + H2O → maltodextrins
(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O

Kit size: 200 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 40 min
Detection limit: 0.5-100% of sample weight
Application examples:
Cereal flours and other materials
Method recognition:
AACC (Method 76-31.01), ICC (Standard No. 164), and RACI (Standard
Method)
Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Only enzymatic kit available
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
参考文献:
An improved enzymic method for the measurement of starch damage in wheat flour. Gibson, T. S., Al Qalla, H. & McCleary, B. V. (1992). Journal of Cereal Science, 15(1), 15-27.
Collaborative evaluation of an enzymatic starch damage assay kit and comparison with other methods. Gibson, T. S., Kaldor, C. J. & McCleary, B. V. (1993). Cereal Chem., 70(1), 47-51.
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Starch properties, in vitro digestibility and sensory evaluation of fresh egg pasta produced from oat, teff and wheat flour. Hager, A. S., Czerny, M., Bez, J., Zannini, E. & Arendt, E. K. (2013). Journal of Cereal Science, 58(1), 156-163.
Effect of sorghum flour composition and particle size on quality properties of gluten-free bread. Trappey, E. F., Khouryieh, H., Aramouni, F. & Herald, T. (2014). Food Science and Technology International, 1082013214523632.
Nutritional properties and ultra-structure of commercial gluten free flours from different botanical sources compared to wheat flours. Hager, A. S., Wolter, A., Jacob, F., Zannini, E. & Arendt, E. K. (2012). Journal of Cereal Science, 56(2), 239-247.
Quality variations in flours used for pretzel manufacturing. Yao, N. & Seetharaman, K. (2010). International Journal of Food Science & Technology, 45(10), 2052-2061.
Effect of corn preparation methods on dry-grind ethanol production by granular starch hydrolysis and partitioning of spent beer solids. Lamsal, B. P., Wang, H. & Johnson, L. A. (2011). Bioresource Technology, 102(12), 6680-6686.
Flaking as a corn preparation technique for dry-grind ethanol production using raw starch hydrolysis. Lamsal, B. P. & Johnson, L. A. (2012). Journal of Cereal Science, 56(2), 253-259.
Chemical composition and functional properties of native chestnut starch (Castanea sativa Mill). Cruz, B. R., Abraão, A. S., Lemos, A. M. & Nunes, F. M. (2013). Carbohydrate Polymers, 94(1), 594-602.
Changes in rice with variable temperature parboiling: thermal and spectroscopic assessment. Himmelsbach, D. S., Manful, J. T. & Coker, R. D. (2008). Cereal chemistry, 85(3), 384-390.
Determination of formulation and processing factors affecting slowly digestible starch, protein digestibility and antioxidant capacity of extruded sorghum–maize composite flour. Licata, R., Chu, J., Wang, S., Coorey, R., James, A., Zhao, Y. & Johnson, S. (2014). International Journal of Food Science & Technology, 49(5), 1408-1419.
Analysis of starch amylolysis using plots for first-order kinetics. Butterworth, P. J., Warren, F. J., Grassby, T., Patel, H. & Ellis, P. R. (2012). Carbohydrate Polymers, 87(3), 2189-2197.